Histone H2A anticorps (AA 1-15)
Aperçu rapide pour Histone H2A anticorps (AA 1-15) (ABIN291582)
Antigène
Voir toutes Histone H2A AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
-
-
Épitope
- AA 1-15
-
Specificité
- A 14 kD band is observed.
-
Homologie
- Percent identity by BLAST analysis: Human, Monkey, Mouse, Rat, Hamster, Elephant, Bovine, Chicken (100%).
-
Purification
- Protein G purified
-
Immunogène
-
This antibody was generated by immunizing rabbits with a mixture of synthetic peptides containing amino acid residues 1-15 (MSGRGKQGGKARAKA) and 81-96 (PRHLQLAIRNDEELNK) of human Histone H2A, GenBank no. ref|NP_066390.1|. Percent identity by BLAST analysis: Human, Monkey, Mouse, Rat, Hamster, Elephant, Bovine, Chicken (100%).
Type of Immunogen: Synthetic peptide -
Isotype
- IgG
-
-
-
-
Indications d'application
-
Approved: IHC, IHC-P (5 μg/mL), WB (2 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. -
Commentaires
-
Target Species of Antibody: Human
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Concentration
- Lot specific
-
Buffer
- PBS, 0.05 % BSA, 0.05 % sodium azide.
-
Agent conservateur
- Sodium azide
-
Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Conseil sur la manipulation
- avoid freeze thaw cycles.
-
Stock
- 4 °C,-20 °C
-
Stockage commentaire
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
-
-
- Histone H2A
-
NCBI Accession
- NP_066390
Antigène
-