DPPA4 anticorps (AA 125-296)

N° du produit ABIN292183

Cet anticorps Chèvre Polyclonal détecte spécifiquement DPPA4 dans WB, ELISA et ICC. Il présente une réactivité envers Souris.

Aperçu rapide pour DPPA4 anticorps (AA 125-296) (ABIN292183)

Antigène: DPPA4 (Developmental Pluripotency Associated 4 (DPPA4))

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp DPPA4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Détail du produit anti-DPPA4 anticorps

Épitope: AA 125-296

Specificité: Selected for its ability to recognize mouse DPPA4 in the applications listed. In direct ELISAs and Western Blots, this antibody shows less than 5 % cross-reactivity with rhDPPA4 and rmDPPA5.

Purification: Affinity purified

Immunogène: E. coli-derived rmDPPA4 (aa125-296).

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, ICC, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse DPPA4. The detection limit for rmDPPA4 is approximately 1 ng/lane under non-reducing and reducing conditions. This antibody has not been optimized for Western blot detection of DPPA4 in cell lysates. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect mouse DPPA4 in mouse embryonic stem cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse DPPA4. The detection limit for rmDPPA4 is approximately 0.2 ng/well.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Détails sur DPPA4

Antigène: DPPA4 (Developmental Pluripotency Associated 4 (DPPA4))

Autre désignation: DPPA4

Sujet: Name/Gene ID: DPPA4

Synonyms: DPPA4

ID gène: 55211

UniProt: Q7L190