HAVCR1 anticorps (AA 200-300)

N° du produit ABIN292372

Cet anticorps Lapin Polyclonal détecte spécifiquement HAVCR1 dans WB, IHC et IHC (p). Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour HAVCR1 anticorps (AA 200-300) (ABIN292372)

Antigène: HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HAVCR1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-HAVCR1 anticorps

Épitope: AA 200-300

Specificité: The amino acid sequence (SPLYSYTTDGNDTVT) used as immunogen for this is 100 % homologous in human, 93 % homologous in vervet/green monkey and 80 % homologous in chimp. Isoforms of different molecular weights have been described for TIM-1. The sequence used for immunogen is conserved between TIM-1 (364aa, NP_036338) and TIM-1 isoform CRA_a (194aa, EAW61615.1). Therefore, the molecular weight observed in western blots may vary depending on the forms present.

Homologie: Percent identity by BLAST analysis: Human, Gorilla (100%) Monkey (93%) Dog (80%).

Purification: Protein G purified

Immunogène: A synthetic peptide (SPLYSYTTDGNDTVT) of human HAVCR1/TIM-1protein were used as immunogen. This sequence corresponds to amino acids (aa) 248-262 of NP_036338 and aa 84-98 of EAW61615.1. Isoforms of HAVCR1/TIM-1 have been described. For example, NP_036338 is 364 aa and EAW61615.1 is 194 aa. EAW61615.1 is referred to as isoform CRA_a. Percent identity by BLAST analysis: Human, Gorilla (100%), Monkey (93%), Dog (80%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (2.5 - 5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5-5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.

Détails sur HAVCR1

Antigène: HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))

Autre désignation: HAVCR1 / KIM-1

Classe de substances: Virus

Sujet: Name/Gene ID: HAVCR1

Synonyms: HAVCR1, HAVCR-1, KIM-1, Kidney injury molecule 1, KIM1, T-cell membrane protein 1, TIM1, TIMD1, TIM-1, HAVCR, TIMD-1

ID gène: 26762

NCBI Accession: NP_036338

UniProt: Q96D42