PAX8 anticorps (AA 2-14)

N° du produit ABIN292477

Cet anticorps Chèvre Polyclonal détecte spécifiquement PAX8 dans WB, IHC, ELISA et IHC (p). Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Chien, Lapin et Singe.

Aperçu rapide pour PAX8 anticorps (AA 2-14) (ABIN292477)

Antigène: PAX8 (Paired Box 8 (PAX8))

Reactivité: Humain, Souris, Rat, Chien, Lapin, Singe

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PAX8 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PAX8 anticorps

Épitope: AA 2-14

Specificité: Human PAX8. This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1)

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Elephant, Rabbit, Opossum, Platypus (100%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide PHNSIRSGHGGLN-C from the N-terminus of human PAX8 (NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Elephant, Rabbit, Opossum, Platypus (100%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur PAX8

Antigène: PAX8 (Paired Box 8 (PAX8))

Autre désignation: PAX8

Sujet: Name/Gene ID: PAX8

Synonyms: PAX8, Paired box 8, Paired domain gene 8, Paired box gene 8, Paired box protein Pax-8

ID gène: 7849

NCBI Accession: NP_003457, NP_039245, NP_039246, NP_039247, NP_054698

UniProt: Q06710

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation