UTF1 anticorps (AA 44-153)

N° du produit ABIN293228

L’anticorps Chèvre Polyclonal anti-UTF1 a été validé pour WB, ELISA et ICC. Il convient pour détecter UTF1 dans des échantillons de Humain.

Aperçu rapide pour UTF1 anticorps (AA 44-153) (ABIN293228)

Antigène: UTF1 (Undifferentiated Embryonic Cell Transcription Factor 1 (UTF1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp UTF1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Détail du produit anti-UTF1 anticorps

Épitope: AA 44-153

Specificité: Selected for its ability to recognize UTF1 in the applications listed.

Purification: Affinity purified

Stérilité: 0.2 μm filtered

Immunogène: E. coli-derived rhUTF1 (aa44-153).

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, ICC, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect UTF1. The detection limit for rhUTF1 is approximately 10 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively. Immunocytochemistry: This antibody can be used at a concentration of 10 μg/mL to detect UTF1 in mouse D3 cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Northern Lights 557-coupled anti-donkey IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect UTF1. The detection limit for rhUTF1 is approximately 1.0 ng/well.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile 40-50 % glycerol, PBS

Concentration: Lot specific

Buffer: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5 % trehalose.

Conseil sur la manipulation: Aviod frequent freeze thaws.

Stock: -20 °C

Stockage commentaire: Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.

Détails sur UTF1

Antigène: UTF1 (Undifferentiated Embryonic Cell Transcription Factor 1 (UTF1))

Autre désignation: UTF1

Sujet: Name/Gene ID: UTF1

Synonyms: UTF1

ID gène: 8433

UniProt: Q5T230