CDH6 anticorps (Extracellular Domain)
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- Antigène Voir toutes CDH6 Anticorps
- CDH6 (K-Cadherin (CDH6))
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Épitope
- Extracellular Domain
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Reactivité
- Humain
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Hôte
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Mouton
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp CDH6 est non-conjugé
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Application
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)
- Specificité
- Recognizes human Cadherin-6. In direct ELISAs and Western Blot, shows less than 5 % cross-reactivity with rhCadherin-8, rhCadherin-11, and rhCadherin-12.
- Purification
- Immunoaffinity purified
- Immunogène
- NS0-derived rhCadherin-6 extracellular domain.
- Isotype
- IgG
- Top Product
- Discover our top product CDH6 Anticorps primaire
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- Indications d'application
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Approved: ELISA (0.5 - 1 μg/mL), Flo (50 μg/mL), ICC (10 μg/mL), WB
Usage: Suitable for use in Western Blot, Immunocytochemistry, Flow Cytometry and ELISA. Western Blot: 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human Cadherin-6. The detection limit is approximately 10 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL to detect human Cadherin-6 in MG63 human osteosarcoma cells cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody for 3 hours at RT followed by donkey anti-sheep lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Flow Cytometry: 50 μg/mL Add 10 μL of the diluted solution to 1-2.5x10^5 MG63 human osteosarcoma cells. This antibody has been tested on MG63 human osteosarcoma cells. Dilute this antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200uL. The binding of unlabeled antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as anti-sheep lgG conjugated to a fluorochrome. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human Cadherin-6. The detection limit for rhCadherin-6 is approximately 1 ng/well. - Commentaires
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile PBS.
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, 5 % trehalose.
- Stock
- -20 °C
- Stockage commentaire
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Short term: -20°C
Long term: -20°C.
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- Antigène
- CDH6 (K-Cadherin (CDH6))
- Autre désignation
- CDH6 / K Cadherin (CDH6 Produits)
- Synonymes
- anticorps KCAD, anticorps K-cadherin, anticorps cad6, anticorps cadherin-6, anticorps CAD6, anticorps c-cad6B, anticorps cadherin 6, anticorps Cdh6, anticorps CDH6
- Sujet
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Name/Gene ID: CDH6
Family: Cadherin
Synonyms: CDH6, K Cadherin, K-cadherin, CAD6, Cadherin-6, Fetal kidney cadherin, KCAD, Kidney cadherin - ID gène
- 1004
- UniProt
- P55285
- Pathways
- Cell-Cell Junction Organization
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