AIRE anticorps (AA 102-115)

N° du produit ABIN293603

L’anticorps anti-AIRE Polyclonal Chèvre est utilisé pour la détection de AIRE dans des échantillons de Humain, Rat, Chien, Cheval et Hamster. Il a été validé pour WB, ELISA, IHC et IHC (p).

Aperçu rapide pour AIRE anticorps (AA 102-115) (ABIN293603)

Antigène: AIRE (Autoimmune Regulator (AIRE))

Reactivité: Humain, Rat, Chien, Cheval, Hamster

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp AIRE est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-AIRE anticorps

Épitope: AA 102-115

Specificité: Human AIRE. This antibody is expected to recognise isoform 1 (NP_000374.1) only.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Rat, Horse, Dog, Hamster, Elephant (100%) Panda (93%) Monkey, Mouse, Bovine (86%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-KDVDLSQPRKGRKP from an internal region of human AIRE (NP_000374.1). Percent identity by BLAST analysis: Human, Gorilla, Rat, Horse, Dog, Hamster, Elephant (100%), Panda (93%), Monkey, Mouse, Bovine (86%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (0.5 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur AIRE

Antigène: AIRE (Autoimmune Regulator (AIRE))

Autre désignation: AIRE

Sujet: Name/Gene ID: AIRE

Synonyms: AIRE, APSI, AIRE1, Autoimmune regulator, APECED, APS1, APECED protein, PGA1

ID gène: 326

NCBI Accession: NP_000374

UniProt: O43918

Pathways: Chromatin Binding