MBD3 anticorps (AA 215-230)

N° du produit ABIN294235

Cet anticorps Souris Monoclonal détecte spécifiquement MBD3 dans WB, IHC et IHC (p). Il présente une réactivité avec des échantillons de Humain, Chien, Boeuf (Vache), Cheval, Porc, Roussette (Chauve-souris), Poulet et Xenopus laevis.

Aperçu rapide pour MBD3 anticorps (AA 215-230) (ABIN294235)

Antigène: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Reactivité: Humain, Chien, Boeuf (Vache), Cheval, Porc, Roussette (Chauve-souris), Poulet, Xenopus laevis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MBD3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 106B691

Détail du produit anti-MBD3 anticorps

Épitope: AA 215-230

Specificité: The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kD and human MBD3 (291 amino acids) is 33 kD. Based on sequence identity, this will detect human, mouse, and rat MBD3 (100 % ), and human and mouse MBD2 (93 %).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus (100%) Marmoset, Mouse, Rat, Hamster, Rabbit, Stickleback, Pufferfish, Zebrafish (94%) Salmon (88%).

Purification: Protein G purified

Immunogène: Amino acids 215-230 (CKAFMVTDEDIRKQEE) of human MBD3 protein were used as the immunogen for this antibody. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus (100%), Marmoset, Mouse, Rat, Hamster, Rabbit, Stickleback, Pufferfish, Zebrafish (94%), Salmon (88%).

Isotype: IgG1

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 μg/mL), WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide. Sourced in TCS.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: avoid freeze thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Détails sur MBD3

Antigène: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Autre désignation: MBD3

Sujet: Name/Gene ID: MBD3

Synonyms: MBD3

ID gène: 53615

UniProt: O95983

Pathways: Chromatin Binding