HAND2 anticorps

N° du produit ABIN294970

Cet anticorps Chèvre Polyclonal détecte spécifiquement HAND2 dans WB, ELISA et IHC. Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour HAND2 anticorps (ABIN294970)

Antigène: HAND2 (Heart and Neural Crest Derivatives Expressed 2 (HAND2))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp HAND2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-HAND2 anticorps

Specificité: Recognizes human HAND2. Species cross-reactivity: Mouse.

Purification: Affinity purified

Stérilité: 0.2 μm filtered

Immunogène: E. coli-derived rhHAND2.

Type of Immunogen: Recombinant protein

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA (0.5 - 1 μg/mL), IHC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.5-1.0 μg/mL. The detection limit for rhHAND2 and rmHAND2 is approximately 1.3 ng/well. Western Blot: 0.1-0.2 μg/mL. The detection limit for rhHAND2 and rmHAND2 is approximately 25 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 10 μg/mL to detect HAND2 in mouse embryos. Cells were fixed with PBS containing 4 % paraformaldehyde and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA. After blocking, cells were incubated with diluted primary antibody followed by NorthernLights 557-coupled anti-goat IgG at RT in the dark. Between each step, cells were washed with PBS containing BSA.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with sterile 40-50 % glycerol, PBS.

Concentration: Lot specific

Buffer: Lyophilized from a 0.2 um filtered solution in PBS, 5 % trehalose.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur HAND2

Antigène: HAND2 (Heart and Neural Crest Derivatives Expressed 2 (HAND2))

Autre désignation: DHAND / HAND2

Sujet: Name/Gene ID: HAND2

Synonyms: HAND2, DHand, DHAND2, Hed, Thing2, BHLHa26

ID gène: 9464

UniProt: P61296

Pathways: Tube Formation, Feeding Behaviour