DPPA5 anticorps

N° du produit ABIN295465

Cet anticorps Chèvre Polyclonal détecte spécifiquement DPPA5 dans ELISA, WB et ICC. Il présente une réactivité envers Souris.

Aperçu rapide pour DPPA5 anticorps (ABIN295465)

Antigène: DPPA5 (Developmental Pluripotency Associated 5 (DPPA5))

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp DPPA5 est non-conjugé

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC)

Détail du produit anti-DPPA5 anticorps

Specificité: Selected for its ability to recognize mouse DPPA5 in the applications listed.

Purification: Affinity purified

Immunogène: E. coli-derived rmDPPA5.

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, ICC, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse DPPA5. The detection limit for rmDPPA5 is approximately 5 ng/lane under non-reducing and reducing conditions. In this format, this antibody shows approximately 25 % cross-reactivity with rhDPPA5 and approximately 10 % cross-reactivity with rmDPPA4. This antibody has not been optimized for Western blot detection of DPPA5 in cell lysates. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect mouse DPPA5 in mouse embryonic stem cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse DPPA5. The detection limit for rmDPPA5 is approximately 0.3 ng/well.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Détails sur DPPA5

Antigène: DPPA5 (Developmental Pluripotency Associated 5 (DPPA5))

Autre désignation: DPPA5

Sujet: Name/Gene ID: DPPA5

Synonyms: DPPA5, ESG-1, HDPPA5, ESG1

ID gène: 340168

UniProt: A6NC42