ERG anticorps (AA 469-481)

N° du produit ABIN295774

Cet anticorps Chèvre Polyclonal détecte spécifiquement ERG dans IHC, ELISA et IHC (p). Il présente une réactivité avec des échantillons de Humain et Singe.

Aperçu rapide pour ERG anticorps (AA 469-481) (ABIN295774)

Antigène: ERG (ERG, ETS transcription factor (ERG))

Reactivité: Humain, Singe

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ERG est non-conjugé

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-ERG anticorps

Épitope: AA 469-481

Specificité: Human ERG. This antibody is expected to recognise both reported isoforms (NP_891548.1, NP_004440.1)

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Horse, Opossum, Platypus, Lizard, Xenopus (92%) Rabbit, Turkey, Chicken (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-PNTRLPTSHMPSH from the C-terminus of human ERG (NP_891548.1, NP_004440.1, NP_001129626.1, NP_001129627.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Horse, Opossum, Platypus, Lizard, Xenopus (92%), Rabbit, Turkey, Chicken (85%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 μg/mL), Peptide-ELISA (1:16000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur ERG

Antigène: ERG (ERG, ETS transcription factor (ERG))

Autre désignation: ERG

Sujet: Name/Gene ID: ERG

Synonyms: ERG, ERG-3, Ets-related, TMPRSS2/ERG fusion, Transcriptional regulator ERG, p55, Transforming protein ERG

ID gène: 2078

NCBI Accession: NP_891548, NP_004440, NP_001129626, NP_001129627

UniProt: P11308