Working dilution: Optimal dilution should be determined by the end user. The following are guidelines only : FC(1-2 μg for 10.6 cells) IHC(4 - 5 μg/mL) WB(0.5 - 1 μg/mL)
Positive control: HeLa cell lysate, rat kidney and rat/mouse testes tissue.
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS, pH 7.4, glycerol 50 % .
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Dilute in PBS or medium that is identical to that used in the assay system.
Stock
-20 °C
Stockage commentaire
Stable for at least one year at -20°C. Avoid multiple freeze-thaw cycles.
FK506-binding proteins, FKBP51 and FKBP52, are high molecular weight immunophilins that are part of the mature glucocorticoid receptor heterocomplex. The N-terminal domains of these proteins bind FK506 and have peptidyl-prolyl isomerase activity that converts prolyl peptide bonds within target proteins from cis- to transproline. The C-terminal domains contain the tetratricopeptide repeats that are involved in protein-protein interactions with Hsp90. Although FKBP52 and FKBP51 share ~75 % sequence similarity, they affect hormone binding by glucocorticoid receptors differently and have different Hsp90-binding characteristics. In addition, FKBP51 is linked to the progesterone receptor while FKBP52 is linked to progesterone, androgen, and glucocorticoid receptors.