FHL1 anticorps

N° du produit ABIN7306298

Cet validé indépendamment anticorps Lapin Polyclonal détecte spécifiquement FHL1 dans WB, IF et IC. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour FHL1 anticorps (ABIN7306298)

Antigène: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FHL1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Détail du produit anti-FHL1 anticorps

Specificité: Recognizes endogenous levels of FHL-1 protein.

Attributs du produit: Rabbit polyclonal antibody to FHL-1

Purification: The antibody was purified by immunogen affinity chromatography.

Immunogène: Recombinant full length protein of human FHL-1

Information d'application

Indications d'application: WB (1:500 - 1:2000), IF/IC (1:50 - 1:100)

Restrictions: For Research Use only

Independent Validation (IF)

Validation #101371 (Immunofluorescence)

by: Alexander Lab, Jacobs School of Medicine and Biomedical Sciences, Department of Medicine, University of Buffalo

No.: #101371

Date: 19.07.2017

Antigène: FHL-1

Numéro du lot: AL1F04A

Application validée: Immunofluorescence

Contrôle positif: MRL/lpr lupus mice (14 weeks of age)

Contrôle négative: FH knockout MRL/lpr lupus mice

Conclusion: Passed. ABIN2966633 works well on murine kidney samples and shows the expected CFH1 localization.

Validation Images

IF staining with ABIN2966633 of kidneys harvested from 14 week old MRL/lpr mice sufficient and deficient in factor H. Cryosections were fixed, stained and images acquired as described in the protocol.

Protocole

Anticorps primaire: ABIN2966633

Anticorps secondaire: goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251)

Full Protocol:

• Prepare kidneys of wt and complement factor H ko MRL/lpr lupus mice of 14 weeks of age.
• Snap-freeze kidneys in 2-methylbutane cooled on dry ice and keep at −80°C until use.
• Sections were cut at 8µm using the cryostat.
• Dry sections for 30min at RT.
• Hydrate sections in PBS 2x for 15min at RT.
• Fix cryostat sections with 4% paraformaldehyde (PFA) for 15min at RT.
• Wash sections 2x with PBS for 15min at RT.
• Incubate sections with primary rabbit anti-FHL-1 antibody (antibodies-online, ABIN2966633, AL1F04A) diluted 1:100 in PBS ON at 4°C.
• Wash sections 2x for 15min with PBS.
• Incubate sections with secondary goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251) diluted 1:100 in PBS for 30min at RT.
• Wash sections 2x for 15min with PBS.
• Incubate sections with ActinGreen 488 ReadyProbes Reagent (ThermoFisher Scientific, R37122, lot 1700569) diluted 1:100 in PBS for 30min at RT.
• Wash sections 2x for 15min with PBS.
• Image acquisition on a Zeiss microscope (Carl Zeiss, Oberkochen, Germany).

Stockage

Format: Liquid

Buffer: Liquid in 0.42 % Potassium phosphate, 0.87 % Sodium chloride, pH 7.3, 30 % glycerol, and 0.01 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Date de péremption: 12 months

Détails sur FHL1

Antigène: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Autre désignation: FHL-1

Sujet: SLIM1, Four and a half LIM domains protein 1, FHL-1, Skeletal muscle LIM-protein 1, SLIM, SLIM-1

ID gène: 2273, 14199, 25177

UniProt: Q13642, P97447, Q9WUH4