Neural Wiskott-Aldrich syndrome protein (WASL) (AA 21-270) anticorps

N° du produit ABIN297874

Cet anticorps Chèvre Polyclonal détecte spécifiquement dans WB, ELISA et ICC. Il présente une réactivité envers Humain.

Aperçu rapide pour Neural Wiskott-Aldrich syndrome protein (WASL) (AA 21-270) anticorps (ABIN297874)

Antigène: Neural Wiskott-Aldrich syndrome protein (WASL)

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Détail du produit

Épitope: AA 21-270

Specificité: Selected for its ability to recognize human N-WASP in the applications listed.

Purification: Affinity purified

Immunogène: E. coli-derived rhN-WASP (aa21-270).

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, ICC, WB

Usage: Western Blot: This antibody can be used at 1.0 μg/mL with the appropriate secondary reagents to detect N-WASP. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect human N-WASP on A431 human epidermoid carcinoma cells stimulated with EGF. Cells were fixed with PBS containing 4 % paraformaldehyde and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA. After blocking, cells were incubated with diluted primary antibody followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark. Between each step, cells were washed with PBS containing BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human N-WASP. The detection limit for rhN-WASP is approximately 1.0 ng/well.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Détail du antigène

Antigène: Neural Wiskott-Aldrich syndrome protein (WASL)

Autre désignation: WASL / N-WASP

Sujet: Name/Gene ID: WASL

Synonyms: WASL, N-WASP, NWASP, Wiskott-Aldrich syndrome-like

ID gène: 8976

UniProt: O00401