CPE anticorps (AA 42-453)

N° du produit ABIN299519

L’anticorps Chèvre Polyclonal anti-CPE a été validé pour WB, ELISA, IP et FACS. Il convient pour détecter CPE dans des échantillons de Humain.

Aperçu rapide pour CPE anticorps (AA 42-453) (ABIN299519)

Antigène: CPE (Carboxypeptidase E (CPE))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CPE est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Détail du produit anti-CPE anticorps

Épitope: AA 42-453

Specificité: Recognizes human Carboxypeptidase E. Cross-reactivity: Shows less than 1 % cross-reactivity with rhCPA1, rmCPA4 and rhCPB1 in ELISA and Western Blot.

Purification: Affinity purified

Immunogène: NS0-derived recombinant human Carboxypeptidase E (aa42-453).

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA (0.1 - 0.2 μg/mL), Flo, IP, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Flow Cytometry. ELISA: 0.1-0.2 μg/mL. Detection limit for rhCPE is ~1 ng/well. Western Blot: 0.1-0.2 μg/mL. Detection limit for rhCPE is ~5 ng/lane under reducing and non-reducing conditions. Flow Cytometry: (A172 cells): For intracellular staining cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μLto 1-5x10^5 cells in a total reaction volume not exceeding 200 μL. ELISA: 0.5-1 μg/mL with the appropriate secondary reagents. The detection limit is ~1 ng/well. Immunoprecipitation: Used to immunoprecipitate rhCPE from conditioned media of transfected NSO cells.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.

Détails sur CPE

Antigène: CPE (Carboxypeptidase E (CPE))

Autre désignation: Carboxypeptidase e / CPE

Sujet: Name/Gene ID: CPE
Subfamily: Metallopeptidase M14
Family: Exopeptidase

Synonyms: CPE, Carboxypeptidase E, CPH, Carboxypeptidase H, Enkephalin convertase

ID gène: 1363

UniProt: P16870

Pathways: Peptide Hormone Metabolism, Synaptic Membrane