alpha Tubulin anticorps (TUBA1) (Biotin)

Details for Product anti-TUBA1 Antibody No. ABIN301997, Fournisseur: Connectez-vous pour afficher
Antigène
  • Tuba1
  • K-ALPHA-1
  • Tuba-1
  • cb944
  • fb22g06
  • tuba1
  • wu:fb22g06
  • Calpha1
  • tubulin, alpha 1A
  • tubulin alpha 1b
  • tubulin, alpha 1b
  • tubulin, alpha 1B
  • Tuba1a
  • TUBA1B
  • tuba1b
  • Tuba1b
Reactivité
Toutes les espèces
297
171
160
59
54
53
44
31
28
28
27
27
26
26
25
24
18
18
18
17
8
8
8
7
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Hôte
Souris
165
146
24
5
2
2
2
1
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp alpha Tubulin est conjugé à/à la Biotin
17
12
10
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
300
125
112
101
95
92
58
39
34
31
13
8
4
3
2
2
2
2
1
1
1
Options
Fournisseur
Connectez-vous pour afficher
N° du produit (Fournisseur)
Connectez-vous pour afficher
Immunogène Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.
Clone TU-01
Isotype IgG1
Specificité The antibody TU-01 recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.
Réactivité croisée (Details) Broad species reactivity
Plasmids, Primers & others Plasmids, Primers & others alpha Tubulin products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation alpha-tubulin (TUBA1 Antibody Extrait)
Sujet The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression.Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.
Pathways Dynamique des Microtubules
Indications d'application Biotinylated antibody is designed for western blotting analysis. Suggested working dilution is 1:1000. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Commentaires

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Do not freeze.
Avoid prolonged exposure to light.
Stock 4 °C
Stockage commentaire Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Images (Fournisseur)
Immunofluorescence (IF) image for anti-alpha Tubulin (TUBA1) antibody (Biotin) (ABIN301997) Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-al...
Immunofluorescence (IF) image for anti-alpha Tubulin (TUBA1) antibody (Biotin) (ABIN301997) Immunofluorescence staining of HeLa human cervix carcinoma cell line using anti-alpha...
Immunofluorescence (IF) image for anti-alpha Tubulin (TUBA1) antibody (Biotin) (ABIN301997) Nucleus is stained with DAPI (blue).
Western Blotting (WB) image for anti-alpha Tubulin (TUBA1) antibody (Biotin) (ABIN301997) Use of anti-alpha-tubulin antibody as a loading control (A) in an Western blotting ex...
Western Blotting (WB) image for anti-alpha Tubulin (TUBA1) antibody (Biotin) (ABIN301997) Use of anti-alpha-tubulin antibody as a loading control (A) in an Western blotting ex...
Western Blotting (WB) image for anti-alpha Tubulin (TUBA1) antibody (Biotin) (ABIN301997) Western blotting analysis of alpha-tubulin in porcine brain lysate by antibody TU-01 ...
Background publications Miyajima, Maruyama, Nonomura, Hatakeyama: "TRIM36 interacts with the kinetochore protein CENP-H and delays cell cycle progression." dans: Biochemical and biophysical research communications, Vol. 381, Issue 3, pp. 383-7, 2009 (PubMed).

Honys, R?nuak, Fecikovua, Jedelskuy, Nebesuarovua, Dobrev, Capkovua: "Cytoskeleton-associated large RNP complexes in tobacco male gametophyte (EPPs) are associated with ribosomes and are involved in protein synthesis, processing, and localization." dans: Journal of proteome research, Vol. 8, Issue 4, pp. 2015-31, 2009 (PubMed).

Bon, Di Carlo, Folgiero, Avetrani, Lazzari, DOrazi, Brizzi, Sacchi, Soddu, Blandino, Mottolese, Falcioni: "Negative regulation of beta4 integrin transcription by homeodomain-interacting protein kinase 2 and p53 impairs tumor progression." dans: Cancer research, Vol. 69, Issue 14, pp. 5978-86, 2009 (PubMed).

Produit citée dans: Lukas, Mazna, Valenta, Doubravska, Pospichalova, Vojtechova, Fafilek, Ivanek, Plachy, Novak, Korinek: "Dazap2 modulates transcription driven by the Wnt effector TCF-4." dans: Nucleic acids research, Vol. 37, Issue 9, pp. 3007-20, 2009 (PubMed).

Smertenko, Blume, Viklický, Dráber: "Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies." dans: European journal of cell biology, Vol. 72, Issue 2, pp. 104-12, 1997 (PubMed).

Smertenko, Blume, Viklický, Opatrný, Dráber: "Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells." dans: Planta, Vol. 201, Issue 3, pp. 349-58, 1997 (PubMed).

Nováková, Dráberová, Schürmann, Czihak, Viklický, Dr-aber: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." dans: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, 1996 (PubMed).

Linhartová, Dráber, Dráberová, Viklický: "Immunological discrimination of beta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins." dans: The Biochemical journal, Vol. 288 ( Pt 3), pp. 919-24, 1993 (PubMed).

Dráber, Dráberová, Viklický: "Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta-tubulin epitope in the sperm head." dans: Histochemistry, Vol. 95, Issue 5, pp. 519-24, 1991 (PubMed).

Dráber, Dráberová, Linhartová, Viklický: "Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies." dans: Journal of cell science, Vol. 92 ( Pt 3), pp. 519-28, 1990 (PubMed).

Dráber, Dráberová, Zicconi, Sellitto, Viklický, Cappuccinelli: "Heterogeneity of microtubules recognized by monoclonal antibodies to alpha-tubulin." dans: European journal of cell biology, Vol. 41, Issue 1, pp. 82-8, 1987 (PubMed).

Grimm, Breitling, Little: "Location of the epitope for the alpha-tubulin monoclonal antibody TU-O1." dans: Biochimica et biophysica acta, Vol. 914, Issue 1, pp. 83-8, 1987 (PubMed).

Viklický, Dráber, Hasek, Bártek: "Production and characterization of a monoclonal antitubulin antibody." dans: Cell biology international reports, Vol. 6, Issue 8, pp. 725-31, 1982 (PubMed).

Avez-vous cherché autre chose?