GFP anticorps (AA 1-100)

N° du produit ABIN3020573

L’anticorps Souris Monoclonal anti-GFP a été validé pour WB et IF. Il convient pour détecter GFP dans des échantillons de Aequorea victoria. Il y a 3+ publications disponibles.

Aperçu rapide pour GFP anticorps (AA 1-100) (ABIN3020573)

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GFP est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-GFP anticorps

Épitope: AA 1-100

Séquence: MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF

Attributs du produit: Tag Antibodies

Purification: Affinity purification

Immunogène: A synthetic peptide corresponding to a sequence within amino acids 1-100 to the N-terminus of GFP protein.

Isotype: IgG

Information d'application

Indications d'application: WB,1:2000 - 1:5000,IF,1:50 - 1:100

Restrictions: For Research Use only

Stockage

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid freeze / thaw cycles

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze / thaw cycles.

Références pour anticorps anti-GFP (ABIN3020573)

Xu, Liu, Zha, Fan, Zhang, Li, Xiao: "A pathogen-derived effector modulates host glucose metabolism by arginine GlcNAcylation of HIF-1α protein." dans: PLoS pathogens, Vol. 14, Issue 8, pp. e1007259, (2019) (PubMed).

Yang, Yang, Sun, Chen, Xu, Lai, Li, Wang, Bhattarai, Xiao, Sun, Zhu, Ma, Adhikari, Sun, Hao, Zhang, Huang, Huang, Jiang, Zhao, Wang, Sun, Yang: "5-methylcytosine promotes mRNA export - NSUN2 as the methyltransferase and ALYREF as an m5C reader." dans: Cell research, Vol. 27, Issue 5, pp. 606-625, (2018) (PubMed).

Zhang, Lu, Liu, Cui, Wang: "Primate-specific miR-603 is implicated in the risk and pathogenesis of Alzheimer's disease." dans: Aging, Vol. 8, Issue 2, pp. 272-90, (2016) (PubMed).

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: GFP

Sujet: The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. It has been used in modified forms to make biosensors, and many animals have been created that express GFP, which demonstrates a proof of concept that a gene can be expressed throughout a given organism, in selected organs, or in cells of interest. GFP can be introduced into animals or other species through transgenic techniques, and maintained in their genome and that of their offspring. To date, GFP has been expressed in many species, including bacteria, yeasts, fungi, fish and mammals, including in human cells.,GFP,GFP tag,GFP-tag,GFP

Poids moléculaire: 27 kDa