Maltose Binding Protein anticorps

N° du produit ABIN3020578

Cet anticorps Souris Monoclonal détecte spécifiquement Maltose Binding Protein dans WB. Il présente une réactivité envers Humain, Souris et Rat et a été mentionné dans 1 publication.

Aperçu rapide pour Maltose Binding Protein anticorps (ABIN3020578)

Antigène: Maltose Binding Protein (MBP)

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Maltose Binding Protein est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Maltose Binding Protein anticorps

Purification: Affinity purification

Immunogène: An MBP-Tag fusion protein

Isotype: IgG

Information d'application

Indications d'application: WB: 1:1000 1:3000

Restrictions: For Research Use only

Stockage

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid freeze / thaw cycles

Stock: -20 °C / -80 °C

Références pour anticorps anti-Maltose Binding Protein (ABIN3020578)

Xin, Zhang, Zhang, Li, Wang, Wang, Wang, Qiu, Song: "CgIL17-5, an ancient inflammatory cytokine in Crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules." dans: Developmental and comparative immunology, Vol. 53, Issue 2, pp. 339-48, (2016) (PubMed).

Détails sur Maltose Binding Protein

Antigène: Maltose Binding Protein (MBP)

Autre désignation: Maltose Binding Protein

Sujet: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein (MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. This antibody recognizes MBP (myelin basic protein) TAG in some expression systems.