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Keratin 10/13 anticorps

Reactivité: Humain, Cat WB, IHC (p), IHC (fro) Hôte: Souris Monoclonal DE-K13 unconjugated
N° du produit ABIN302093
  • Antigène
    Keratin 10/13
    Reactivité
    • 11
    • 11
    • 1
    Humain, Cat
    Hôte
    • 11
    Souris
    Clonalité
    • 11
    Monoclonal
    Conjugué
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Inconjugué
    Application
    • 11
    • 11
    • 9
    • 4
    • 2
    • 2
    • 2
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificité
    The mouse monoclonal antibody DE-K13 reacts with cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins, represented in epithelial tissues. DE-K13 recognizes only cytokeratin 13 on formalin-fixed, paraffin-embedded tissue sections.
    Réactivité croisée (Details)
    Human, Feline (Cat), Other species Not tested
    Purification
    Purified by protein-A affinity chromatography.
    Pureté
    > 95 % (by SDS-PAGE)
    Immunogène
    Cytoskeletal preparation extracted from human ectocervical epithelium.
    Clone
    DE-K13
    Isotype
    IgG2a
  • Indications d'application
    Immunohistochemistry (paraffin sections): Standard ABC technique (DAB+), recommended dilution: 10 μg/mL (1:100), positive tissue: tonsil. Pretreatment: 0.1 % pepsin in 0.1 M HCl for 30 min at room temperature or Target Retrieval Solution - High pH . The antibody DE-K13 reacts exclusively with Cytokeratin 13.
    Immunohistochemistry (frozen sections): Use at an assay dependent dilution.
    Western blotting: recommended dilution: 1-3 μg/mL.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C. Do not freeze.
  • Kozaki, Nakamura, Iguchi, Kano, Watanabe, Fujiwara, Hasegawa: "Immunohistochemical analysis of cytokeratin expression in dog skin." dans: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 63, Issue 1, pp. 1-4, (2001) (PubMed).

    Ivanyi, Minke, Hageman, Groeneveld, van Doornewaard, Misdorp: "Cytokeratins as markers of initial stages of squamous metaplasia in feline mammary carcinomas." dans: American journal of veterinary research, Vol. 54, Issue 7, pp. 1095-102, (1993) (PubMed).

    Ivanyi, Minke, Hageman, Groeneveld, van Doornewaard: "Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells." dans: American journal of veterinary research, Vol. 53, Issue 3, pp. 304-14, (1992) (PubMed).

  • Antigène
    Keratin 10/13
    Autre désignation
    Cytokeratin 10+13
    Sujet
    Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratins 10 and 13 both belong to type I family (acidic cytokeratins).,K10, K13, CK10, CK13, CK-10, CD13, CYK10, CYK13, KRT10, KRT13
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