Clathrin Heavy Chain (CLTC) anticorps

N° du produit ABIN302098

Cet anticorps Souris Monoclonal détecte spécifiquement dans WB, ELISA, FACS, IP et ICC. Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache) et Porc et a été mentionné dans 1 publication.

Aperçu rapide pour Clathrin Heavy Chain (CLTC) anticorps (ABIN302098)

Antigène: Clathrin Heavy Chain (CLTC)

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: BF-06

Détail du produit

Fonction: Anti-Clathrin Heavy Chain Purified

Specificité: The antibody BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa intracellular protein involved in receptor-mediated endocytosis.

Réactivité croisée (Details): Human, Porcine, Mouse, Rat, Bovine, Other not determined

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Pureté: > 95 % (by SDS-PAGE)

Isotype: IgM

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 4-10 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Référence

Mac?rek, Zíková, Dráberová, Dráber: "Monoclonal antibody BF-06 against the heavy chain of clathrin." dans: Folia biologica, Vol. 49, Issue 6, pp. 238-40, (2004) (PubMed).

Détail du antigène

Antigène: Clathrin Heavy Chain (CLTC)

Autre désignation: Clathrin Heavy Chain

Sujet: Clathrin heavy chain,Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis.,CHC, CLHC, MRD56

ID gène: 1213

UniProt: Q00610