TIMP3 anticorps (AA 175-211)

N° du produit ABIN3023959

Cet anticorps anti-TIMP3 est un anticorps Lapin Polyclonal détectant TIMP3 dans WB, IP, FACS, IF et IHC (p). Adapté pour Humain, Souris, Rat, Boeuf (Vache), Chien et Cheval.

Aperçu rapide pour TIMP3 anticorps (AA 175-211) (ABIN3023959)

Antigène: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cheval

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TIMP3 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-TIMP3 anticorps

Épitope: AA 175-211

Purification: Protein A affinity chromatography

Immunogène: Amino acids 175-211 of human TIMP3 Ab was used as the immunogen for this antibody (100% homologous in cow, dog and horse, and 94% homologous in mouse and rat).

Isotype: IgG

Information d'application

Indications d'application:

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP3 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: use antibody at 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IP: 1-2 μg/500 μg protein lysate,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the TIMP3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur TIMP3

Antigène: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Autre désignation: TIMP3

Sujet: TIMP3 (tissue inhibitor of metalloproteinases 3), along with family members TIMP1, TIMP2, and TIMP4, are inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix (ECM). An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. TIMP's inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes.

ID gène: 7078