Keratin Basic anticorps

N° du produit ABIN3023995

Cet anticorps anti- est un anticorps Souris Monoclonal détectant dans WB, IF, FACS et IHC (p). Adapté pour Humain, Rat, Souris, Lapin, Boeuf (Vache), Poulet, Singe et Chien.

Aperçu rapide pour Keratin Basic anticorps (ABIN3023995)

Antigène: Keratin Basic

Reactivité: Humain, Rat, Souris, Lapin, Boeuf (Vache), Poulet, Singe, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AE3

Détail du produit

Purification: Protein G purified

Immunogène: Solubilized cytokeratin extract from human stratum corneum was used as the immunogen.

Isotype: IgG1

Information d'application

Indications d'application: Differences in protocols and secondaries may cause the Basic Cytokeratin antibody to need titering for optimal performance.

1. FFPE staining requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Basic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détail du antigène

Antigène: Keratin Basic

Autre désignation: Basic Cytokeratin

Sujet: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This antibody recognizes basic (Type II or HMW) cytokeratins, which include 67  kDa (CK1), 64  kDa (CK3), 59  kDa (CK4), 58  kDa (CK5), 56  kDa (CK6), 52  kDa (CK8). Many studies have shown the usefulness of keratin markers in cancer research and tumor diagnosis. The basic family clone AE3 is commonly used in combination with clone AE1 as a pan cytokeratin antibody acidic + basic cocktail (< a href=../pan-cytokeratin-antibody-cocktail-ae1-ae3-v2330>Cat No V2330).

ID gène: 3848