Keratin Basic anticorps

N° du produit ABIN3023995

Cet anticorps anti-Keratin Basic Monoclonal Souris (Clone AE3) (ABIN3023995) détecte spécifiquement Keratin Basic dans WB, IHC (p), IF et FACS. L’anticorps est réactif avec des échantillons de Humain, Rat et Souris.

Aperçu rapide pour Keratin Basic anticorps (ABIN3023995)

Antigène: Keratin Basic

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Carrier-free

Clone: AE3

Détail du produit

Fonction: Basic Cytokeratin Antibody (azide and preservative free)

Purification: Protein G purified

Immunogène: Solubilized cytokeratin extract from human stratum corneum was used as the immunogen.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Differences in protocols and secondaries may cause the Basic Cytokeratin antibody to need titering for optimal performance.

1. FFPE staining requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Basic Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détail du antigène

Antigène: Keratin Basic

Autre désignation: Basic Cytokeratin

Sujet: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This antibody recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8). Many studies have shown the usefulness of keratin markers in cancer research and tumor diagnosis. The basic family clone AE3 is commonly used in combination with clone AE1 as a pan cytokeratin antibody acidic + basic cocktail (< a href=../pan-cytokeratin-antibody-cocktail-ae1-ae3-v2330>Cat No V2330).