pan Keratin anticorps (PE)

N° du produit ABIN3023996

Cet anticorps Souris Monoclonal détecte spécifiquement pan Keratin dans IF et FACS. Il présente une réactivité envers Humain, Rat, Souris, Boeuf (Vache), Singe, Chien, Lapin et Poulet.

Aperçu rapide pour pan Keratin anticorps (PE) (ABIN3023996)

Antigène: pan Keratin (panKRT)

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Singe, Chien, Lapin, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp pan Keratin est conjugé à/à la PE

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: AE1-AE3

Détail du produit anti-pan Keratin anticorps (PE)

Pureté: Protein G affinity chromatography

Immunogène: Human epidermal keratin was used as the immunogen for this pan Cytokeratin antibody.

Isotype: IgG1

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the pan Cytokeratin antibody AE1 + AE3 to be titered up or down for optimal performance.\. Flow Cytometry : 5 μL/test/million cells or 5 μL/test/100 μL of whole blood,Immunofluorescence : 1:50-1:100 for 30 minutes at RT (1)

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: 500 μL at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the pan Cytokeratin antibody at 2-8°C. Conjugate is light sensitive, store in the dark.

Détails sur pan Keratin

Antigène: pan Keratin (panKRT)

Autre désignation: Pan Cytokeratin

Sujet: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This pan keratin antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3-6, CK8, CK10, CK14-16, and CK19. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis. The AE1 + AE3 antibody cocktail is a broad spectrum pan cytokeratin antibody cocktail which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. It stains cytokeratin present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.