pan Keratin anticorps

N° du produit ABIN3024005

Cet anticorps Souris Monoclonal détecte spécifiquement pan Keratin dans IF, FACS, WB et IHC (p). Il présente une réactivité envers Humain, Rat, Souris, Boeuf (Vache), Singe, Chien, Lapin et Poulet.

Aperçu rapide pour pan Keratin anticorps (ABIN3024005)

Antigène: pan Keratin (panKRT)

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Singe, Chien, Lapin, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp pan Keratin est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PAN-CK (Cocktail)

Détail du produit anti-pan Keratin anticorps

Purification: Protein G affinity chromatography

Immunogène: Human epidermal keratin was used as the immunogen for the pan Cytokeratin antibody cocktail.

Isotype: IgG kappa

Information d'application

Indications d'application: Optimal dilution of the pan Cytokeratin antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the pan Cytokeratin antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur pan Keratin

Antigène: pan Keratin (panKRT)

Autre désignation: Pan Cytokeratin

Sujet: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67  kDa (CK1), 64  kDa (CK3), 59  kDa (CK4), 58  kDa (CK5), 56  kDa (CK6), 55  kDa (CK7), 52  kDa (CK8), 56.5  kDa (CK10), 53  kDa (CK13), 50  kDa (CK14), 50  kDa (CK15), 48  kDa (CK16), 46  kDa (CK17), 45  kDa (CK18) and 40  kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.