TYR anticorps (AA 233-247)

N° du produit ABIN3024010

Cet anticorps anti-TYR Monoclonal Souris (Clone T311) (ABIN3024010) détecte spécifiquement TYR dans IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour TYR anticorps (AA 233-247) (ABIN3024010)

Antigène: TYR (Tyrosinase (TYR))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TYR est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: T311

Détail du produit anti-TYR anticorps

Épitope: AA 233-247

Fonction: Tyrosinase Antibody

Séquence: IPYWDWRDAE KCDIC

Purification: Protein G affinity chromatography

Immunogène: Recombinant Tyrosinase protein was used as the immunogen for this antibody. Subsequent testing has localized the epitope to within amino acids 233-247 (IPYWDWRDAEKCDIC) (see Citation 12).

Isotype: IgG2a, kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Tyrosinase antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur TYR

Antigène: TYR (Tyrosinase (TYR))

Autre désignation: Tyrosinase

Sujet: Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway, the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients.