CD1a anticorps

N° du produit ABIN3024177

L’anticorps Souris Monoclonal anti-CD1a a été validé pour FACS, IF et IHC (p). Il convient pour détecter CD1a dans des échantillons de Humain.

Aperçu rapide pour CD1a anticorps (ABIN3024177)

Antigène: CD1a

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD1a est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 66IIC7

Détail du produit anti-CD1a anticorps

Purification: Protein G affinity chromatography

Immunogène: Human thymocytes were used as the immunogen for the CD1a antibody.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal dilution of the CD1a antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the CD1a antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur CD1a

Antigène: CD1a

Autre désignation: CD1a

Sujet: At least five CD1 genes (CD1a, b, c, d, and e) are identified. CD1 proteins have been demonstrated to restrict T cell response to non-peptide lipid and glycolipid antigens and play a role in non-classical antigen presentation. CD1a is a non-polymorphic MHC Class 1 related cell surface glycoprotein, expressed in association with Beta-2 microglobulin. Anti-CD1a labels Langerhans cell histiocytosis (Histiocytosis X), extranodal histiocytic sarcoma, a subset of T-lymphoblastic lymphoma/leukemia, and interdigitating dendritic cell sarcoma of the lymph node. When combined with antibodies against TTF-1 and CD5, anti-CD1a is useful in distinguishing between pulmonary and thymic neoplasms since CD1a is consistently expressed in thymic lymphocytes in both typical and atypical thymomas, but only focally in 1/6 of thymic carcinomas and not in lymphocytes in pulmonary neoplasms. Anti-CD1a is reported to be a new marker for perivascular epithelial cell tumor (PEComa).

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process