Melanoma anticorps

N° du produit ABIN3024241

Cet anticorps anti-Melanoma est un anticorps Souris Monoclonal détectant Melanoma dans IHC (p), IF et FACS. Adapté pour Humain et Cheval.

Aperçu rapide pour Melanoma anticorps (ABIN3024241)

Antigène: Melanoma

Reactivité: Humain, Cheval

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Melanoma est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: NKI-beteb

Détail du produit anti-Melanoma anticorps

Aucune reactivité croisée: Rat (Rattus)

Purification: Protein G affinity chromatography

Immunogène: Membranes from a human melanoma metastasis were used as the immunogen for this Melanoma antibody (gp100).

Isotype: IgG2b kappa

Information d'application

Indications d'application: Differences in protocols and secondaries may require the gp100 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Melanoma antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Melanoma

Antigène: Melanoma

Sujet: By immunohistochemistry, this melanoma gp100 antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. The melanoma antibody labels formalin/paraffin melanomas and other tumors showing melanocytic differentiation.

ID gène: 6490