CD15 anticorps

N° du produit ABIN3024288

L’anticorps Souris Monoclonal anti-CD15 a été validé pour FACS, IF et IHC (p). Il convient pour détecter CD15 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour CD15 anticorps (ABIN3024288)

Antigène: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD15 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: FUT4-815

Détail du produit anti-CD15 anticorps

Purification: PEG precipitation

Immunogène: Purified human neutrophils were used as the immunogen for the CD15 antibody.

Isotype: IgM kappa

Information d'application

Indications d'application: Optimal dilution of the CD15 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the CD15 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur CD15

Antigène: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Autre désignation: CD15

Sujet: CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95 % of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.