CD45RA anticorps

N° du produit ABIN3024414

L’anticorps Souris Monoclonal anti-CD45RA a été validé pour FACS, IF et IHC (p). Il convient pour détecter CD45RA dans des échantillons de Humain.

Aperçu rapide pour CD45RA anticorps (ABIN3024414)

Antigène: CD45RA

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD45RA est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PTPRC-1131

Détail du produit anti-CD45RA anticorps

Purification: Protein G affinity chromatography

Immunogène: Stimulated human leukocytes were used as immunogen for this CD45RA antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Due to variation in protocol and secondary antibody used, the CD45RA antibody may need to be titered for optimal performance.1. FFPE staining requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells,Immunofluorescence: 1-2 μg/mL,Immunohistology (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: The CD45RA antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Détails sur CD45RA

Antigène: CD45RA

Autre désignation: CD45RA

Sujet: CD45RA is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50 % of peripheral CD4+ T-cells, 50 % of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).