CD45RB anticorps

N° du produit ABIN3024427

Cet anticorps anti-CD45RB est un anticorps Souris Monoclonal détectant CD45RB dans FACS, IF, WB et IHC (p). Adapté pour Humain.

Aperçu rapide pour CD45RB anticorps (ABIN3024427)

Antigène: CD45RB

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD45RB est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PD7-26

Détail du produit anti-CD45RB anticorps

Purification: Protein G purified

Immunogène: Human peripheral blood lymphocytes maintained in T cell growth factor were used as the immunogen for this CD45RB antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Variations in protocols, secondaries and substrates may require the CD45RB antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western Blot: 0.5-1 μg/mL, FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the CD45RB antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur CD45RB

Antigène: CD45RB

Autre désignation: CD45RB

Sujet: CD45RB is one of the four major isoforms of CD45.  The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved.  Clone PD7/26 antibody is specific to CD45RB, and stains most leukemias and lymphomas. This CD45RB antibody is often used in a cocktail with clone 2B11, which will bind to all CD45 isoforms (< a href=../cd45-antibody-cocktail-2b11-pd726-v2243>Cat No V2243).

ID gène: 5788