CD99 anticorps

N° du produit ABIN3024516

L’anticorps Souris Monoclonal anti-CD99 a été validé pour FACS, WB, IF et IHC (p). Il convient pour détecter CD99 dans des échantillons de Humain et Rat.

Aperçu rapide pour CD99 anticorps (ABIN3024516)

Antigène: CD99

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD99 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: HO36-1-1

Détail du produit anti-CD99 anticorps

Purification: PEG precipitation

Immunogène: Purified E-rosette forming cells from human peripheral blood lymphocytes were used as the immunogen for the CD99 antibody.

Isotype: IgM kappa

Information d'application

Indications d'application: Optimal dilution of the CD99 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL, Flow Cytometry: 5-10 μL/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the CD99 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur CD99

Antigène: CD99

Autre désignation: CD99

Sujet: CD99 is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. [UniProt]