FBXO5 anticorps (N-Term)

N° du produit ABIN3024714

L’anticorps anti-FBXO5 Monoclonal Souris est utilisé pour la détection de FBXO5 dans des échantillons de Humain. Il a été validé pour WB et IHC (p).

Aperçu rapide pour FBXO5 anticorps (N-Term) (ABIN3024714)

Antigène: FBXO5 (F-Box Protein 5 (FBXO5))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FBXO5 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Classe de qualité: Carrier-free

Clone: FBXP5

Détail du produit anti-FBXO5 anticorps

Épitope: N-Term

Fonction: EMI-1 Antibody (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogène: A human partial recombinant N-terminal protein fragment was used as the immunogen for the EMI-1 antibody.

Isotype: IgG2a, kappa

Information d'application

Indications d'application: Optimal dilution of the EMI-1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the EMI-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur FBXO5

Antigène: FBXO5 (F-Box Protein 5 (FBXO5))

Autre désignation: EMI-1

Sujet: Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI-1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of EMI-1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

UniProt: Q9UKT4

Pathways: Mitotic G1-G1/S Phases