FBXO5 anticorps (AA 1-250)

N° du produit ABIN3024715

Cet anticorps anti-FBXO5 Monoclonal Souris (Clone EMI1-1176) (ABIN3024715) détecte spécifiquement FBXO5 dans WB et IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour FBXO5 anticorps (AA 1-250) (ABIN3024715)

Antigène: FBXO5 (F-Box Protein 5 (FBXO5))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FBXO5 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: EMI1-1176

Détail du produit anti-FBXO5 anticorps

Épitope: AA 1-250

Fonction: EMI1 Antibody

Purification: Protein G affinity chromatography

Immunogène: A recombinant fragment (203 amino acid residues between aa 1-250) from the human protein was used as the immunogen for the EMI1 antibody.

Isotype: IgG2a, kappa

Information d'application

Indications d'application: Optimal dilution of the EMI1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the EMI1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur FBXO5

Antigène: FBXO5 (F-Box Protein 5 (FBXO5))

Autre désignation: EMI1

Sujet: Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of EMI1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

UniProt: Q9UKT4

Pathways: Mitotic G1-G1/S Phases