FBXO5 anticorps (AA 1-250)

N° du produit ABIN3024717

Détail du produit anti-FBXO5 anticorps

Antigène: FBXO5 (F-Box Protein 5 (FBXO5))

Épitope: AA 1-250

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FBXO5 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogène: A recombinant fragment (203 amino acid residues between aa 1-250) from the human protein was used as the immunogen for the EMI1 antibody.

Clone: EMI1-1176

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal dilution of the EMI1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the EMI1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur FBXO5

Antigène: FBXO5 (F-Box Protein 5 (FBXO5))

Autre désignation: EMI1 (FBXO5 Produits)

Synonymes: anticorps EMI1, anticorps FBX5, anticorps Fbxo31, anticorps 2510044I10Rik, anticorps C85305, anticorps Emi1, anticorps emi1, anticorps fc65h02, anticorps wu:fc65h02, anticorps wu:fe06e07, anticorps wu:fz79f03, anticorps zgc:136397, anticorps zgc:158541, anticorps fbx5, anticorps Fbxo5, anticorps ACYPI007854, anticorps fbxo5, anticorps F-box protein 5, anticorps F-box only protein 5, anticorps F-box protein 5 L homeolog, anticorps F-box protein 5 S homeolog, anticorps FBXO5, anticorps Fbxo5, anticorps fbxo5, anticorps fbxo5.L, anticorps fbxo5.S

Sujet: Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of EMI1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Pathways: Mitotic G1-G1/S Phases