EpCAM anticorps

N° du produit ABIN3024720

L’anticorps Souris Monoclonal anti-EpCAM a été validé pour WB, FACS, IF et IHC (p). Il convient pour détecter EpCAM dans des échantillons de Souris et Rat.

Aperçu rapide pour EpCAM anticorps (ABIN3024720)

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivité: Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EpCAM est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: Epcam-1158

Détail du produit anti-EpCAM anticorps

Aucune reactivité croisée: Humain

Pureté: Unpurified high titer supernatant

Immunogène: Recombinant full-length rat protein was used as the immunogen for the rat EpCAM antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal dilution of the rat EpCAM antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The epitopes of mAbs Epcam/1158 and < a href=../tds/rat-epcam-antibody-epcam-1159-v3118s>Epcam/1159 monoclonal antibodies are different.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 5-10 μL/million cells in 0.1ml,Immunofluorescence: 1:50-1:100,Western blot: 1:100-1:200,Immunohistochemistry (FFPE): 1:50-1:100 30 min at RT (1),Prediluted format: incubate for 30 min at RT (3)

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Bioreactor concentrate with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: Store the rat EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur EpCAM

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Autre désignation: EpCAM

Sujet: EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.