EpCAM anticorps (Cytoplasmic Domain)

N° du produit ABIN3024725

Cet anticorps Souris Monoclonal détecte spécifiquement EpCAM dans WB, FACS, IF et IHC (p). Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour EpCAM anticorps (Cytoplasmic Domain) (ABIN3024725)

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EpCAM est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: EGP40-1110

Détail du produit anti-EpCAM anticorps

Épitope: Cytoplasmic Domain

Purification: Protein G affinity chromatography

Immunogène: A recombinant human protein fragment from the cytoplasmic domain was used as the immunogen for the EpCAM antibody.

Isotype: IgG2b kappa

Information d'application

Indications d'application: Optimal dilution of the EpCAM antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur EpCAM

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Autre désignation: EpCAM

Sujet: EGP40 is a 40-43  kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.