EpCAM anticorps (AA 27-59)

N° du produit ABIN3024734

Cet anticorps Souris Monoclonal détecte spécifiquement EpCAM dans WB et IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour EpCAM anticorps (AA 27-59) (ABIN3024734)

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EpCAM est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Classe de qualité: Carrier-free

Clone: SPM528

Détail du produit anti-EpCAM anticorps

Épitope: AA 27-59

Fonction: EpCAM Antibody (azide and preservative free)

Aucune reactivité croisée: Rat (Rattus)

Purification: Protein G affinity chromatography

Immunogène: Neuraminidase treated GLS-1 human small cell lung carcinoma cells were used as the immunogen for this EpCAM antibody. The epitope has been localized to amino acids 27-59.

Isotype: IgG1, kappa

Information d'application

Indications d'application: The optimal dilution of the EpCAM antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur EpCAM

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Autre désignation: EpCAM

Sujet: EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

UniProt: P16422