Fibronectin anticorps

N° du produit ABIN3024767

Cet anticorps Souris Monoclonal détecte spécifiquement Fibronectin dans IF, FACS et IHC (p). Il présente une réactivité envers Humain.

Aperçu rapide pour Fibronectin anticorps (ABIN3024767)

Antigène: Fibronectin

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Fibronectin est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 568

Détail du produit anti-Fibronectin anticorps

Attributs du produit: Epitope of this mAb is located in the 8th type III repeat in the cell-binding region of fibronectin. Fibronectins are disulfide-linked, dimeric glycoproteins of ~440 kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This mAb is excellent for staining of formalin-fixed, paraffin-embedded tissues.

Purification: Protein G affinity chromatography

Immunogène: High molecular weight proteins secreted by cultivated human fibroblasts were used as the immunogen for the Fibronectin antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal dilution of the Fibronectin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30-60 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Fibronectin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Fibronectin

Antigène: Fibronectin

Sujet: Epitope of this mAb is located in the 8th type III repeat in the cell-binding region of fibronectin. Fibronectins are disulfide-linked, dimeric glycoproteins of ~440  kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This mAb is excellent for staining of formalin-fixed, paraffin-embedded tissues.