Nkx2-2 anticorps

N° du produit ABIN3024826

Cet anticorps anti-Nkx2-2 est un anticorps Souris Monoclonal détectant Nkx2-2 dans IF, FACS et IHC (p). Adapté pour Humain, Souris, Rat et Poulet.

Aperçu rapide pour Nkx2-2 anticorps (ABIN3024826)

Antigène: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Reactivité: Humain, Souris, Rat, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nkx2-2 est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NX2-294

Détail du produit anti-Nkx2-2 anticorps

Purification: Protein G affinity chromatography

Immunogène: Human NKX2.2 recombinant protein was used as the immunogen for this antibody.

Isotype: IgG2b kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NKX2.2 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the NKX2.2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Nkx2-2

Antigène: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Autre désignation: NKX2.2

Sujet: Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. antibody to NKX2.2 detects a valuable marker for Ewing sarcoma, with a sensitivity of 93 % and a specificity of 89 %, and aids in the differential diagnosis of small round cell tumors.

ID gène: 4821

Pathways: Dopaminergic Neurogenesis