Fibronectin anticorps

N° du produit ABIN3024852

L’anticorps Souris Monoclonal anti-Fibronectin a été validé pour IF, FACS et IHC (p). Il convient pour détecter Fibronectin dans des échantillons de Humain, Rat, Souris et Porc.

Aperçu rapide pour Fibronectin anticorps (ABIN3024852)

Antigène: Fibronectin

Reactivité: Humain, Rat, Souris, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Fibronectin est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: TV-1

Détail du produit anti-Fibronectin anticorps

Purification: Protein G affinity chromatography

Immunogène: A T-cell lymphoma biopsy was used as the immunogen for this fibronectin antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Fibronectin antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Fibronectin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Fibronectin

Antigène: Fibronectin

Sujet: Fibronectin is a soluble dimeric glycoprotein of 440  kDa, which is present in cells, extracellular matrix, and blood. There are two types of fibronectin: soluble fibronectin which is produced by hepatocytes and found in blood plasma, and insoluble cellular fibronectin which is produced by fibroblasts in a soluble form and latter assembled into an insoluble form via fibril formation.

This antibody reacts with the cellular as well as plasma form of fibronectin. Reportedly, after iv administration, this antibody localizes to tumor vessels where it binds to the underlying basement. The Fibronectin epitope recognized by this antibody is not accessible in normal tissues to the circulating antibody indicating that it can be used to specifically target tumor vessels in vivo. Clone TV-1 fibronectin antibody is reportedly useful for delivering vasoactive agents to tumors to induce increased vascular permeability or blood flow prior to treatment with chemotherapeutic drugs or antibodies.

ID gène: 2335