Involucrin anticorps (AA 421-568)

N° du produit ABIN3024971

Cet anticorps Souris Monoclonal détecte spécifiquement Involucrin dans IF, FACS et IHC (p). Il présente une réactivité envers Humain, Chien, Porc, Gorilla et Owl Monkey.

Aperçu rapide pour Involucrin anticorps (AA 421-568) (ABIN3024971)

Antigène: Involucrin (IVL)

Reactivité: Humain, Chien, Porc, Gorilla, Owl Monkey

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Involucrin est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM259

Détail du produit anti-Involucrin anticorps

Épitope: AA 421-568

Aucune reactivité croisée: Souris

Purification: Protein G affinity chromatography

Immunogène: Purified involucrin from human keratinocytes was used as the immunogen for this Involucrin antibody. Its epitope maps between amino acids 421-568 of human involucrin.

Isotype: IgG1 kappa

Information d'application

Indications d'application: The optimal dilution of the Involucrin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by digestion with Trypsin or Protease XXV at 1 mg/mL PBS for 5 min at 37oC. Enzyme digestion is better than citrate buffer based epitope unmasking.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.1-0.2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Involucrin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Involucrin

Antigène: Involucrin (IVL)

Autre désignation: Involucrin

Sujet: Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.