Cytokeratin 7 anticorps

N° du produit ABIN3025125

L’anticorps anti-Cytokeratin 7 Monoclonal Souris est utilisé pour la détection de Cytokeratin 7 dans des échantillons de Humain. Il a été validé pour IF, FACS et IHC (p).

Aperçu rapide pour Cytokeratin 7 anticorps (ABIN3025125)

Antigène: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 7 est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: KRT7-760

Détail du produit anti-Cytokeratin 7 anticorps

Attributs du produit: It recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Purification: Protein G affinity chromatography

Immunogène: Recombinant full-length human KRT7 protein was used as the immunogen for the Cytokeratin 7 antibody.

Isotype: IgG1

Information d'application

Indications d'application: Optimal dilution of the Cytokeratin 7 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Cytokeratin 7 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Cytokeratin 7

Antigène: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Autre désignation: Cytokeratin 7

Sujet: It recognizes an intermediate filament protein (IFP) of 55  kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).