Laminin gamma 1 anticorps

N° du produit ABIN3025186

Cet anticorps anti-Laminin gamma 1 est un anticorps Rat Monoclonal détectant Laminin gamma 1 dans IF, IHC (p) et FACS. Adapté pour Humain et Souris.

Aperçu rapide pour Laminin gamma 1 anticorps (ABIN3025186)

Antigène: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Laminin gamma 1 est non-conjugé

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: A5

Détail du produit anti-Laminin gamma 1 anticorps

Purification: Protein G affinity chromatography

Immunogène: A murine EHS laminin preparation was used as the immunogen for the Laminin gamma 1 antibody.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal dilution of the Laminin gamma 1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Laminin gamma 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Laminin gamma 1

Antigène: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Autre désignation: Laminin gamma 1

Sujet: Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. This mAb reacts with laminin B2/1 chain of ~210  kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific mAbs. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This mAb stains uniformly all human and murine basement membranes.