MLANA anticorps

N° du produit ABIN3025225

Cet anticorps anti-MLANA est un anticorps Souris Monoclonal détectant MLANA dans IF, FACS et IHC (p). Adapté pour Humain et Cheval.

Aperçu rapide pour MLANA anticorps (ABIN3025225)

Antigène: MLANA (Melan A (MLANA))

Reactivité: Humain, Cheval

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MLANA est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM342

Détail du produit anti-MLANA anticorps

Aucune reactivité croisée: Souris, Rat (Rattus)

Purification: Protein G affinity chromatography

Immunogène: Recombinant human protein was used as the immunogen for this anti-Melan-A antibody.

Isotype: IgG2b kappa

Information d'application

Indications d'application: The optimal dilution of the anti-Melan-A antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the anti-Melan-A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur MLANA

Antigène: MLANA (Melan A (MLANA))

Autre désignation: Melan-A

Sujet: Melan-A/MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1/Melan-A is present in melanosomes and endoplasmic reticulum. This mAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.