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Calponin anticorps

Reactivité: Humain, Rat IHC (p), IF, FACS Hôte: Souris Monoclonal CNN1-832-CALP unconjugated
N° du produit ABIN3025300
  • Antigène Tous les produits Calponin
    Calponin
    Reactivité
    • 38
    • 32
    • 24
    • 8
    • 8
    • 7
    • 5
    • 3
    • 1
    • 1
    • 1
    Humain, Rat
    Hôte
    • 24
    • 15
    Souris
    Clonalité
    • 27
    • 12
    Monoclonal
    Conjugué
    • 24
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Calponin est non-conjugé
    Application
    • 18
    • 17
    • 17
    • 12
    • 11
    • 10
    • 9
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)
    Purification
    Protein G affinity chromatography
    Immunogène
    Recombinant full-length human protein (CNN1/832) and crude human uterus extract (CALP) were used as the immunogen for the Calponin antibody cocktail.
    Clone
    CNN1-832-CALP
    Isotype
    IgG
  • Indications d'application
    Optimal dilution of the Calponin antibody cocktail should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    0.2 mg/mL
    Buffer
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store the Calponin antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Antigène
    Calponin
    Abstract
    Calponin Produits
    Synonymes
    anticorps CalPoNin, anticorps cpn-1, anticorps cpn-2
    Sujet
    Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34  kDa form and a 29  kDa form. Expression of the 29  kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this mAb reacts with only the 34  kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
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