Calponin anticorps (full length)

N° du produit ABIN3025300

Cet anticorps anti-Calponin Monoclonal Souris (Clone CNN1-832-CALP) (ABIN3025300) détecte spécifiquement Calponin dans IHC (p), IF et FACS. L’anticorps est réactif avec des échantillons de Humain et Rat.

Aperçu rapide pour Calponin anticorps (full length) (ABIN3025300)

Antigène: Calponin

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Calponin est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: CNN1-832-CALP

Détail du produit anti-Calponin anticorps

Épitope: full length

Fonction: Calponin Antibody Cocktail

Séquence: CALP

Purification: Protein G affinity chromatography

Immunogène: Recombinant full-length human protein (CNN1/832) and crude human uterus extract (CALP) were used as the immunogen for the Calponin antibody cocktail.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal dilution of the Calponin antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Calponin antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur Calponin

Antigène: Calponin

Sujet: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this mAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

UniProt: P51911