Myogenin anticorps

N° du produit ABIN3025322

Cet anticorps Souris Monoclonal détecte spécifiquement Myogenin dans WB, IF, FACS et IHC (p). Il présente une réactivité envers Humain, Souris, Rat, Porc et Cat.

Aperçu rapide pour Myogenin anticorps (ABIN3025322)

Antigène: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Reactivité: Humain, Souris, Rat, Porc, Cat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Myogenin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MGN185

Détail du produit anti-Myogenin anticorps

Purification: Protein G affinity chromatography

Immunogène: Human myogenin recombinant protein was used as the immunogen for this antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Myogenin antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Myogenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Myogenin

Antigène: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Autre désignation: Myogenin

Sujet: Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80 % amino acid homology in their bHLH motifs. Myogenin antibody labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms' tumor.

ID gène: 4656

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development