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Neurofilament (Light Chain) anticorps

L’anticorps Souris Monoclonal anti- a été validé pour IHC (p) et IF. Il convient pour détecter dans des échantillons de Humain et Rat.
N° du produit ABIN3025338

Aperçu rapide pour Neurofilament (Light Chain) anticorps (ABIN3025338)

Antigène

Neurofilament (Light Chain)

Reactivité

  • 2
  • 2
  • 1
Humain, Rat

Hôte

  • 1
  • 1
Souris

Clonalité

  • 1
  • 1
Monoclonal

Conjugué

  • 2
Inconjugué

Application

  • 2
  • 2
  • 1
  • 1
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone

MSI27-1
  • Purification

    Protein G affinity chromatography

    Immunogène

    Recombinant human neurofilament light chain protein was used as the immunogen for the Neurofilament antibody.

    Isotype

    IgG1
  • Indications d'application

    Optimal dilution of the Neurofilament antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    Restrictions

    For Research Use only
  • Concentration

    1 mg/mL

    Buffer

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    Agent conservateur

    Azide free

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store the Neurofilament antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Antigène

    Neurofilament (Light Chain)

    Sujet

    Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. [RefSeq]
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