PAX6 anticorps (N-Term)

N° du produit ABIN3025374

L’anticorps anti-PAX6 Monoclonal Souris est utilisé pour la détection de PAX6 dans des échantillons de Humain. Il a été validé pour IHC (p).

Aperçu rapide pour PAX6 anticorps (N-Term) (ABIN3025374)

Antigène: PAX6 (Paired Box 6 (PAX6))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PAX6 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PAX6-1166

Détail du produit anti-PAX6 anticorps

Épitope: AA 1-300, N-Term

Fonction: PAX6 Antibody

Purification: Protein G affinity chromatography

Immunogène: A recombinant fragment (N-terminus, aa 1-300) was used as the immunogen for the PAX6 antibody.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal dilution of the PAX6 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the PAX6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur PAX6

Antigène: PAX6 (Paired Box 6 (PAX6))

Autre désignation: PAX6

Sujet: Pax genes contain paired domains with strong homology to genes in Drosophila, which are involved in programming early development. Lesions in the Pax-6 gene account for most cases of aniridia, a congenital malformation of the eye, chiefly characterized by iris hypoplasia, which can cause blindness. Pax-6 is involved in other anterior segment malformations besides aniridia, such as Peters anomaly, a major error in the embryonic development of the eye with corneal clouding with variable iridolenticulocorneal adhesions. The Pax-6 gene encodes a transcriptional regulator that recognizes target genes through its paired-type DNA-binding domain. The paired domain is composed of two distinct DNA-binding subdomains, the amino-terminal subdomain and the carboxy-terminal subdomain, which bind respective consensus DNA sequences. The human Pax-6 gene produces two alternatively spliced isoforms that have the distinct structure of the paired domain.

UniProt: P26367

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis